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Mark Harmon plays Special Agent Leroy Jethro Gibbs, a former Marine Gunnery Sergeant. He is an experienced criminal investigator and a highly-skilled interrogator. Gibbs' often unorthodox method and willingness to bend the rules always gets the job done.
Michael Weatherley stars as Agent Anthony DiNozzo, a streetwise Special Agent working under Gibbs for NCIS. A former Baltimore Homicide Detective, Tony is tough and knows how to work the system. He has sharp instincts and a frat-boy sense of humour.
Perrette plays NCIS crime lab technician, Abby, a "Jane of all trades" - from DNA processing to fingerprint and document analysis, she does it all. She has a Goth-chick attitude and tattoos to match, but takes her job as a lab tech seriously.
De Pablo stars in NCIS as Officer Ziva David, a Mossad agent assigned to Gibbs' team by Director Shepard after she assisted NCIS in locating and terminating a rogue Mossad agent.
Rocky Carroll moves from recurring guest star to full time cast member this season as NCIS Assistant Director Leon Vance. The move coincides with Vance's promotion to Director following the murder of Director Shepard.
David McCallum plays Dr. Donald "Ducky" Mallard, a medical examiner with the Naval Criminal Investigative Service. As a result of constantly dealing with death, he has developed a somewhat dark sense of humour.
Sean Murray plays Timothy McGee, a naive Special Agent for the NCIS team. He has tremendous respect for Special Agent Gibbs. He is an intelligent agent with a specific talent for computers, but awkwardness in social situations.